By Paul L. Dubin
The fast improvement of recent packings for aqueous size-exclusion chromatography has revolutionized this box. excessive solution non-adsorptive columns now make attainable the effective separation of proteins and the quick and particular selection of the molecular weight distribution of artificial polymers. This expertise is additionally being utilized to the separation of small ions, the characterization of associating platforms, and the dimension of branching. even as, basic reviews are elucidating the mechanisms of a number of the chromatographicprocesses. those advancements in rules and purposes are assembled for the 1st time during this publication. basic matters are handled: the jobs of pore constitution and macromolecular dimensions, hydrophobic and electrostatic results, and the selection and regulate of column potency. High-performance packings in response to derivatized silica are reviewed intimately. designated strategies are completely defined, together with SEC/LALLS, inverse exclusion chromatography, and frontal quarter chromatography.
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Extra resources for Aqueous Size-Exclusion Chromatography
In this chapter the term "hydrophobic interaction" is defined from an operational viewpoint, as any favorable nonpolar association of solutes in aqueous media irrespective of the underlying energetics. Interactions between solvent, solute, and gel will be examined from this viewpoint to clarify the nature of the hydrophobic behavior of the concentrated hydrophilic gels, which are well known but not fully elucidated. Nature of hydrophobicity of hydrophilic gels Many hydrophilic gels are known, under certain conditions, to exhibit strong hydrophobicity that seriously affects chromatographic performance, but the nature and origin of this hydrophobicity are not well understood.
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