Read e-book online Bacterial and Bacteriophage Genetics: An Introduction PDF

By Edward A. Birge

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When placed under the appropriate set of conditions, growth of the original cells in the culture is prevented so that only those cells which have acquired one or more new characteristics via genetic exchange (recombinants) will be able to grow and divide. Each recombinant cell ultimately divides many times to produce a colony, a macroscopically visible pile of cells on the surface of an agar plate. Some examples of kinds of selective agents which have been used to prevent growth of the parental cells are antibiotics, bacteriophages, and required nutrients.

On the structure of the folded chromosome of Eseheriehia eoli. Journal of Mo1ecular Bio1ogy 71: 127-147. Chapter 2 The Laws of Probability and Their Application to Prokaryote Cultures The preceding chapter included discussions of some of the problems associated with the analysis of prokaryotic genetic systems. An additional theoretical problem which must be considered is that of sampling. As noted earlier, it is not generally possible to recover all of the progeny from a cross, due to the necessity of using some sort of selective technique to find a few recombinant individuals among many parental types.

Where N is the number of items in the population and n is the number of items in the sampie. The frequency distribution of all possible combinations of successes and failures within sampies of a certain size is given by the binomial expansion (a + br, where a is the proportion of successes in the total population, b is the proportion of failures, and n is the sampie size. Since this quantity is somewhat difficult to evaluate, an approximation method is generally used. , where r is the number of successes in the sampie, e is the base of the naturallogarithms, and m = np.

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