Read e-book online Bacterial Persistence: Methods and Protocols PDF

By Jan Michiels, Maarten Fauvart

This quantity provides a accomplished choice of equipment which have been instrumental to the present realizing of bacterial persisters. Chapters within the booklet hide themes starting from normal equipment for measuring persister degrees in Escherichia coli cultures, protocols for the decision of the persister subpopulation in Candida albicans, quantitative measurements of kind I and sort II persisters utilizing ScanLag, to in vitro and in vivo models for the research of the intracellular job of antibiotics. Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and pointers on troubleshooting and warding off recognized pitfalls.

Authoritative and state of the art, Bacterial patience: tools and Protocols brings jointly the main revered researchers in bacterial patience whose reports will stay very important to knowing this box for a few years to come.

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C) Samples of 5 μL are transferred from wells of row A to row B using the replicator strip. (d) Pins of the replicator strip are decontaminated by immersing into bleach and rinsed twice with water. Between each step, the excess liquid is absorbed into paper towels. Finally, the pins are immersed into ethanol and flame sterilized. (e) Transfer of 5 μL aliquots from the wells of the previous row to the next row is repeated. The replicator is bleached/flame sterilized between each round of dilution.

J Mol Biol 332 (4):809–819 39. Christensen SK, Gerdes K (2004) Delayedrelaxed response explained by hyperactivation of RelE. Mol Microbiol 53(2):587–597 40. Gonzalez Barrios AF, Zuo R, Hashimoto Y, Yang L, Bentley WE, Wood TK (2006) Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorumsensing regulator (MqsR, B3022). J Bacteriol 188(1):305–316 41. Yamaguchi Y, Inouye M (2011) Regulation of growth and death in Escherichia coli by toxinantitoxin systems. Nat Rev Microbiol 9 (11):779–790.

If antibiotic is supplied at the very moment of inoculation of the culture [5], in the lag phase, or in the early logarithmic phase then the surviving persisters belong to type I. At early stages of growth, the number of persisters does not increase (Fig. 1b). Type I persisters are lost in the course of repeated cycles of dilution and growth into early log phase [3]. Type II persisters are formed when a growing culture starts approaching to the stationary phase. When nutrients are depleting and growth conditions deteriorate, their number is progressively increasing and they make up a great majority of the persister fraction [3] (Fig.

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